›› 2011, Vol. 23 ›› Issue (3): 161-165.doi: 10.3969/j.issn.1004-616x.2011.03.001

• 论著 •     Next Articles

Expressing the alteration of DNA repair genes in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate

LI Ying1，WANG Quan-kai1，2，WANG An-na1，2，HU Jie1，XU Jian-ning1，2，*   

  1. 1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050; 2. Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
  • Received:2010-12-30 Revised:2011-03-10 Online:2011-05-30 Published:2011-05-30
  • Contact: XU Jian-ning

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Abstract: To observe the expression change of malignant transformation- related DNA repair genes in human bronchial epithelial cells (16HBE) induced by glycidyl methacrylate (GMA). METHODS：The expression of DNA repair genes in 30th generation cells which had malignant cell phenotype was evaluated with the gene chip of “human genome stability/DNA repair microarray”. RESULTS：Compared with control cells in the 30th generation，eight genes screened from 113 genes showed different expressions in cells induced by GMA. Among the eight genes，there were 7 genes (NBN，RAD50，RAD51，OGG1，XAB2，TOP3A，TNKS) with increased expression (ratio>2)and 1 gene (NEIL2) with lower expression (0

Key words: glycidyl methacrylate, human bronchial epithelial cells, cell malignant transformation, DNA repair genes, gene expression